Clinical Immunology

Disease-Modifying Therapies

Disease-Modifying Therapies

What Do You Need to Know?

  • Treatment goal: NEDA-3 (no relapses, no disability progression, no MRI activity); trend toward early high-efficacy therapy (EHET) over escalation
  • Natalizumab: anti-α4-integrin; highest PML risk (JCV+, prior immunosuppression, >24 months); ~68% relapse reduction; severe rebound on discontinuation
  • Ocrelizumab: anti-CD20; only PPMS-approved DMT (ORATORIO trial); risk of hypogammaglobulinemia and HBV reactivation
  • Fingolimod: S1P modulator; first-dose bradycardia (6-hour cardiac monitoring); macular edema; VZV risk; rebound on stopping
  • Alemtuzumab: anti-CD52; secondary autoimmunity (thyroid 30–40%, ITP, anti-GBM nephritis); monthly monitoring for 4 years
  • Teriflunomide: Category X teratogen; requires cholestyramine washout before conception
  • Glatiramer acetate: safest DMT in pregnancy; no washout required
  • Dimethyl fumarate: Nrf2 pathway; PML risk if sustained lymphopenia (ALC <500 for >6 months)
Treatment Strategy

Escalation vs Early High-Efficacy Therapy (EHET)

ApproachStrategyWhen to Consider
Escalation Start moderate-efficacy DMT (interferon, glatiramer, teriflunomide, dimethyl fumarate); escalate if breakthrough disease Low relapse rate, low lesion burden, favorable prognostic features, older age, mild CIS
Early high-efficacy therapy (EHET) Start with high-efficacy DMT (natalizumab, ocrelizumab, alemtuzumab, cladribine) from diagnosis High relapse rate, high lesion burden, brainstem/spinal cord lesions, young age, poor prognostic features
  • Trend shifting toward EHET — observational data suggest better long-term outcomes when high-efficacy therapy started early
  • No RCTs directly comparing escalation vs EHET — evidence from observational registries (MSBase, SUMMIT)

NEDA-3 (No Evidence of Disease Activity)

ComponentDefinition
No relapsesNo clinical attacks over assessment period
No disability progressionNo confirmed EDSS worsening sustained for 3–6 months
No MRI activityNo new/enlarging T2 lesions and no Gd-enhancing lesions
  • NEDA-4 adds brain volume loss (<0.4%/year) as fourth measure (not yet standard in practice)
💎 Board Pearl
  • NEDA-3 is the current treatment target — no relapses + no disability progression + no MRI activity
  • Failure to achieve NEDA-3 should prompt consideration of switching to a higher-efficacy DMT
Injectable DMTs (Moderate Efficacy)

Interferons & Glatiramer Acetate

DrugMechanismEfficacyKey Side EffectsMonitoring
IFN-β1a (Avonex — IM weekly; Rebif — SC 3×/wk) Immunomodulatory: ↓ T-cell activation, ↓ BBB permeability, Th1 → Th2 shift, antiviral ~30% relapse reduction Flu-like symptoms, injection site reactions, hepatotoxicity, depression, leukopenia CBC, LFTs q3–6 months; neutralizing antibodies if breakthrough
IFN-β1b (Betaseron, Extavia — SC QOD) Same as IFN-β1a ~30% Same; slightly more injection site reactions Same
Peginterferon-β1a (Plegridy — SC q2 weeks) Pegylated IFN-β1a; longer half-life ~36% Same as interferons; less frequent dosing Same
Glatiramer acetate (Copaxone — SC daily or 40 mg 3×/wk) Synthetic MBP analog; induces Th2 shift; generates regulatory T cells ~29% Injection site reactions, lipoatrophy, immediate post-injection reaction (chest tightness, flushing — benign, self-limited; occurs in ~10%) None required routinely; safest DMT in pregnancy
💎 Board Pearl
  • Glatiramer acetate is the safest DMT in pregnancy — no washout required; often used as bridge therapy
  • Interferons: check neutralizing antibodies if breakthrough disease on therapy — up to 25–45% develop NAbs
  • Interferons and glatiramer are moderate-efficacy (~30% relapse reduction) — adequate for mild disease, not for aggressive MS
Oral DMTs

S1P Receptor Modulators

DrugSelectivityEfficacyKey Side EffectsMonitoring
Fingolimod (Gilenya — 0.5 mg daily) Non-selective S1P1/3/4/5 ~54% relapse reduction First-dose bradycardia/AV block (6-hr cardiac monitoring); macular edema; VZV reactivation; PML (rare); rebound on discontinuation First-dose 6-hr ECG; ophthalmology 3–4 months; CBC; VZV titer pre-treatment (vaccinate if seronegative)
Siponimod (Mayzent) Selective S1P1/S1P5 Approved for active SPMS (EXPAND trial) Similar to fingolimod; requires CYP2C9 genotyping (contraindicated in *3/*3 homozygotes) CYP2C9 genotype before starting; first-dose cardiac monitoring; ophthalmology
Ozanimod (Zeposia) Selective S1P1/S1P5 High Less cardiac risk than fingolimod; no first-dose observation (gradual titration); MAO-B inhibition → serotonin syndrome risk with SSRIs CBC; cardiac evaluation if indicated; ophthalmology
Ponesimod (Ponvory) Selective S1P1 Superior to teriflunomide (OPTIMUM trial) Gradual 14-day dose titration; similar class effects Cardiac evaluation; CBC; ophthalmology; LFTs

Other Oral Agents

DrugMechanismEfficacyKey Side EffectsMonitoring
Teriflunomide (Aubagio — 14 mg daily) Inhibits DHODH → ↓ pyrimidine synthesis → ↓ lymphocyte proliferation ~30% (moderate) Category X teratogen; hepatotoxicity; hair thinning; diarrhea; peripheral neuropathy LFTs monthly × 6 months; pregnancy test; cholestyramine washout required before conception (drug persists up to 2 years without washout)
Dimethyl fumarate (Tecfidera — 240 mg BID) Activates Nrf2 antioxidant pathway; depletes memory T cells ~49% Flushing, GI upset (take with food); PML risk if ALC <500 sustained >6 months CBC q6 months; hold if ALC <500 for >6 months
Diroximel fumarate (Vumerity) Same active metabolite as dimethyl fumarate Same Better GI tolerability; otherwise same Same as dimethyl fumarate
Cladribine (Mavenclad) Purine analog → selective T and B cell depletion (immune reconstitution therapy) ~58% Lymphopenia; herpes zoster; theoretical malignancy risk; teratogenic CBC before each course; 2 short courses/year × 2 years then no ongoing therapy needed
💎 Board Pearl
  • Fingolimod first dose = 6 hours cardiac monitoring — S1P agonism causes transient bradycardia; vaccinate for VZV first
  • Siponimod requires CYP2C9 genotyping — contraindicated in *3/*3 homozygotes; approved for active SPMS
  • Teriflunomide is Category X — requires cholestyramine washout; verify undetectable drug levels before conception
  • DMF/PML risk: sustained ALC <500 for >6 months → discontinue; monitor CBC q6 months
  • Cladribine is immune reconstitution therapy (IRT) — 2 short courses then done; no continuous treatment
  • Fingolimod rebound: severe disease reactivation 2–6 months after discontinuation; bridge or overlap with next DMT
Infusion DMTs (High Efficacy)

Anti-CD20 Therapies (B-Cell Depletion)

DrugRoute/DosingEfficacyKey Side EffectsMonitoring
Ocrelizumab (Ocrevus) IV q6 months ~47% relapse reduction; only PPMS-approved DMT (ORATORIO trial) Infusion reactions; infections; HBV reactivation; hypogammaglobulinemia; possibly slight ↑ breast cancer risk HBV screening; immunoglobulin levels; CBC; breast cancer screening
Ofatumumab (Kesimpta) SC monthly (self-administered) ~51% (ASCLEPIOS trials) Injection site reactions; infections; HBV reactivation HBV screening; immunoglobulin levels
Ublituximab (Briumvi) IV q6 months (1-hour infusion) ~51% (ULTIMATE trials) Infusion reactions; infections; similar to other anti-CD20s HBV screening; immunoglobulin levels
Rituximab (off-label) IV q6 months Widely used off-label; strong real-world evidence Same class effects; less expensive than ocrelizumab Same as other anti-CD20s
  • Anti-CD20 agents deplete CD20+ B cells but spare plasma cells (CD20-negative) and pro-B cells
  • B-cell depletion is near-complete within 2 weeks and lasts 6 months
  • Long-term use can lead to hypogammaglobulinemia → increased infection risk; monitor IgG levels

Other High-Efficacy Infusion Agents

DrugMechanismEfficacyKey Side EffectsMonitoring
Natalizumab (Tysabri — IV q4 weeks) Anti-α4-integrin (VLA-4) → blocks lymphocyte migration across BBB ~68% relapse reduction (AFFIRM trial); among highest efficacy PML (JCV reactivation); infusion reactions; hepatotoxicity; severe rebound on discontinuation JCV antibody + index q6 months; MRI q3–6 months for PML surveillance
Alemtuzumab (Lemtrada — 5 days yr 1, 3 days yr 2) Anti-CD52 → pan-lymphocyte depletion (T, B, NK, monocytes); immune reconstitution therapy ~55%; superior to IFN-β1a (CARE-MS I/II) Secondary autoimmunity: thyroid (30–40%), ITP (2–3%), anti-GBM nephritis (0.3%); infusion reactions; infections CBC, TSH, creatinine, urinalysis monthly for 4 years after last dose
Mitoxantrone (rarely used) Topoisomerase II inhibitor; immunosuppressive High Cardiotoxicity (cumulative dose limit 140 mg/m²); therapy-related AML; blue urine/sclera LVEF before each dose; lifetime cumulative limit; rarely used now due to toxicity
💎 Board Pearl
  • Ocrelizumab is the ONLY FDA-approved DMT for PPMS (ORATORIO trial) — the single most tested DMT fact on boards
  • Anti-CD20 agents spare plasma cells — they are CD20-negative; this is why OCBs persist despite B-cell depletion
  • Natalizumab has the highest single-agent relapse reduction (~68%) but carries highest PML risk
  • Alemtuzumab is immune reconstitution therapy — 2 courses then done; but monitoring continues 4+ years for secondary autoimmunity
  • Alemtuzumab autoimmunity: thyroid disease (Graves most common, 30–40%), ITP, anti-GBM nephritis
PML Risk & Natalizumab Stratification

PML Risk Stratification

JCV StatusPrior ImmunosuppressionDuration >24 monthsEstimated PML Risk
JCV antibody negativeAnyAny~0.1/1,000 (very low)
JCV antibody positive, index ≤0.9No<24 months~0.1/1,000
JCV antibody positive, index >1.5No>24 months~6–13/1,000
JCV antibody positive, index >1.5Yes>24 months~13/1,000 (highest)
  • Recheck JCV antibody q6 months — seroconversion at ~2–3%/year
  • Extended-interval dosing (EID): q6–8 weeks instead of q4 weeks may reduce PML risk while maintaining efficacy

PML with Other DMTs

DMTPML RiskMechanism
NatalizumabHighest (~1:800 overall; up to 13:1,000 in high-risk)Blocks immune surveillance of CNS by preventing T-cell entry
FingolimodRare (much lower than natalizumab)Lymphocyte sequestration in lymph nodes
Dimethyl fumarateRare; linked to sustained lymphopeniaOnly if ALC <500 sustained >6 months
OcrelizumabVery rare (mostly carry-over from prior natalizumab)B-cell depletion alone rarely causes PML

PML Diagnosis & IRIS

  • PML presentation: subacute progressive focal deficits (hemiparesis, cognitive changes, visual field cuts); no enhancement on MRI initially
  • MRI: multifocal, asymmetric white matter lesions involving subcortical U-fibers; NO enhancement; NO mass effect
  • Diagnosis: CSF JCV PCR (sensitivity ~80%; can be negative early)
  • Treatment: discontinue natalizumab; PLEX to accelerate drug clearance; supportive care
  • PML-IRIS (immune reconstitution inflammatory syndrome): paradoxical worsening 1–4 weeks after natalizumab cessation as immune cells flood back into CNS; enhancement appears on MRI; treat with steroids; high morbidity
💎 Board Pearl
  • Natalizumab PML risk triad: JCV+, prior immunosuppression, duration >24 months — risk is multiplicative
  • PML on MRI: subcortical U-fiber white matter lesions, NO enhancement, NO mass effect — distinguishes from MS relapse
  • PML-IRIS: paradoxical worsening after stopping natalizumab; MRI shows new enhancement; treat with high-dose steroids
  • JCV antibody seroconversion occurs at ~2–3%/year — recheck every 6 months on natalizumab
Switching & Sequencing DMTs

Key Switching Considerations

ScenarioApproachKey Concern
Natalizumab → anti-CD20Start anti-CD20 within 4–6 weeks of last natalizumab doseMinimize gap to prevent rebound disease; monitor for PML/IRIS during transition
Natalizumab → fingolimodStart fingolimod within 4–8 weeksLonger washout ↑ rebound risk; shorter washout ↑ additive immunosuppression risk
Fingolimod → any DMTWait for lymphocyte recovery (ALC >500–800); usually 4–8 weeksSevere rebound disease 2–6 months after stopping fingolimod; overlap or bridge
Dimethyl fumarate → any DMTCan switch relatively quickly; ensure ALC recoveringIf ALC was sustained <500, wait for recovery before starting immunosuppressive DMT
Alemtuzumab → any DMTTypically only if relapses recur after immune reconstitutionWait for lymphocyte recovery; continue autoimmune monitoring regardless

Washout Periods

DMTWashoutNotes
Interferons/GlatiramerNone neededCan switch immediately; no significant immunosuppressive carryover
TeriflunomideCholestyramine 8 g TID × 11 days (or activated charcoal)Without washout, drug persists up to 2 years; verify plasma level <0.02 mg/L
Fingolimod4–8 weeks for lymphocyte recoverySevere rebound possible — bridge if needed
Natalizumab4–6 weeks (minimize gap)Rebound disease peaks at 3–6 months post-discontinuation
Anti-CD20Wait for B-cell reconstitution (6–12+ months)Rarely needed; most patients stay on anti-CD20 long-term
💎 Board Pearl
  • Natalizumab rebound: severe disease reactivation peaks 3–6 months after stopping; minimize washout gap
  • Fingolimod rebound: severe relapses 2–6 months after discontinuation; bridge or overlap with next DMT
  • Teriflunomide washout: cholestyramine 8 g TID × 11 days; without it, drug persists up to 2 years
Pregnancy & DMT Safety

DMT Pregnancy Safety Profile

DMTPregnancy CategoryWashout Before ConceptionKey Notes
Glatiramer acetateCompatibleNone requiredSafest DMT in pregnancy; can continue until positive pregnancy test; often used as bridge
InterferonsLikely compatibleDiscontinue at conceptionReassuring safety data; some centers continue until positive pregnancy test
NatalizumabUse with cautionCase-by-caseMay continue through 3rd trimester in highly active disease (risk of rebound > fetal risk); neonatal hematologic effects
Dimethyl fumarateInsufficient dataDiscontinue; short half-lifeLimited human data; discontinue when planning conception
FingolimodContraindicated2 months washoutTeratogenic in animal studies; risk of rebound during washout
TeriflunomideCategory XCholestyramine washout mandatoryMost dangerous in pregnancy; drug persists up to 2 years without washout; verify undetectable levels
CladribineContraindicated6 months after last doseTeratogenic; wait ≥6 months after last dose
Anti-CD20sAvoid near deliveryDiscontinue; wait for B-cell recoveryIgG crosses placenta → neonatal B-cell depletion; typically stop 6+ months before conception
AlemtuzumabAvoid4 months after last doseSecondary autoimmunity risk to mother continues regardless
💎 Board Pearl
  • Glatiramer acetate = safest DMT in pregnancy — no washout needed; often first choice for women planning conception
  • Teriflunomide = Category X — mandatory cholestyramine washout before conception; confirm undetectable plasma levels
  • MS relapse rate ↓ 3rd trimester, ↑ postpartum — consider resuming DMT immediately after delivery
  • Both men and women on teriflunomide should undergo washout before conceiving (drug is present in semen)
Symptomatic Management

Treatment by Symptom

SymptomFirst-LineAlternatives
FatigueAmantadine 100 mg BIDModafinil 200 mg daily; exercise; sleep hygiene; treat depression
SpasticityBaclofen (oral or intrathecal pump)Tizanidine; diazepam; dantrolene; botulinum toxin for focal spasticity
Neuropathic painGabapentin; pregabalinDuloxetine; TCAs; carbamazepine for trigeminal neuralgia
Bladder (urgency)Oxybutynin; tolterodineMirabegron; intermittent catheterization if PVR >100 mL; onabotulinum toxin injection
Walking impairmentDalfampridine (4-AP) 10 mg BIDK+ channel blocker; improves speed in ~35%; check renal function; seizure risk
Paroxysmal symptomsCarbamazepine (low dose)Oxcarbazepine; gabapentin; treats ephaptic transmission
DepressionSSRIs/SNRIsCBT; lifetime prevalence ~50% in MS
TremorNo highly effective treatmentIsoniazid (with B6); clonazepam; DBS for severe cases
💎 Board Pearl
  • Dalfampridine (4-aminopyridine) is the only FDA-approved drug for MS walking impairment — blocks K+ channels in demyelinated axons
  • Intrathecal baclofen pump for severe spasticity not responsive to oral agents
  • Paroxysmal symptoms respond to low-dose carbamazepine — not treating seizures, treating ephaptic transmission
  • Amantadine for MS fatigue — also has anti-glutamatergic properties; often first-line

References

  • Rae-Grant A, Day GS, Marrie RA, et al. Practice guideline recommendations summary: disease-modifying therapies for adults with multiple sclerosis. Neurology. 2018;90(17):777-788.
  • Hauser SL, Bar-Or A, Comi G, et al. Ocrelizumab versus interferon beta-1a in relapsing multiple sclerosis (OPERA I/II). N Engl J Med. 2017;376(3):221-234.
  • Montalban X, Hauser SL, Kappos L, et al. Ocrelizumab versus placebo in primary progressive multiple sclerosis (ORATORIO). N Engl J Med. 2017;376(3):209-220.
  • Polman CH, O'Connor PW, Havrdova E, et al. A randomized, placebo-controlled trial of natalizumab for relapsing multiple sclerosis (AFFIRM). N Engl J Med. 2006;354(9):899-910.
  • Kappos L, Bar-Or A, Cree BAC, et al. Siponimod versus placebo in secondary progressive multiple sclerosis (EXPAND). Lancet. 2018;391(10127):1263-1273.
  • Coles AJ, Twyman CL, Arnold DL, et al. Alemtuzumab for patients with relapsing multiple sclerosis (CARE-MS II). Lancet. 2012;380(9856):1829-1839.
  • Confavreux C, Hutchinson M, Hours MM, et al. Rate of pregnancy-related relapse in multiple sclerosis (PRIMS). N Engl J Med. 1998;339(5):285-291.