Clinical Immunology

Last Minute Review

Neuroimmunology — Last Minute Review

A last-minute review of high-yield facts — dense tables and one-liners for RITE/Board prep. Not a substitute for the full notes.
McDonald Criteria (2024 Revision) — MS Diagnosis
Criterion2024 RequirementHow to Fulfill
DIS (Dissemination in Space)≥1 T2 lesion in ≥2 of 5 CNS regionsPeriventricular, cortical/juxtacortical, infratentorial, spinal cord, optic nerve (NEW) — need ≥2 of 5 areas
DIT (Dissemination in Time)Not eliminated as a concept — can be satisfied by additional biomarkers at the time of a single clinical/radiographic eventDIT can be met by: new T2/Gd+ lesion on follow-up MRI, simultaneous Gd+ and non-enhancing lesions, or biomarkers (OCBs, kappa FLC, ≥6 central vein sign lesions, or paramagnetic rim lesions) at the time of a single event
RIS (Radiologically Isolated Syndrome)Qualifies as MS ONLY when DIS is met AND there is additional supportive evidence, with no clinical or radiographic alternativeRequires: DIS fulfilled + (DIT shown on serial MRI, OR ≥2 CSF-specific OCBs, OR ≥6 central vein sign lesions / paramagnetic rim lesions) + no better alternative diagnosis

Key 2024 vs 2017 Changes

Feature2017 Criteria2024 Criteria
DIS regions4 regions (periventricular, cortical/juxtacortical, infratentorial, spinal cord)5 regions — optic nerve added as 5th location (via MRI orbits, OCT, or VEP)
DIT requirementStrictly required for diagnosisRemoved as strict requirement — diagnosis possible after single event if other evidence supports
New biomarkersOCBs onlyOCBs + central vein sign, paramagnetic rim lesions, kappa free light chains (CSF)
RISNot diagnostic — must await symptomsCan qualify for MS diagnosis if DIS + supportive biomarkers
Special populationsLimited guidanceSpecific guidance for children and adults ≥50 years
CIS categoryCIS = first event, not yet MSMany former CIS cases now diagnosable as MS at first presentation
DIS Region (5 total)Evidence
Periventricular≥1 T2 lesion (same threshold as 2017)
Cortical/juxtacortical≥1 lesion
Infratentorial≥1 lesion
Spinal cord≥1 lesion
Optic nerve (NEW)MRI optic nerve lesion, abnormal OCT (RNFL thinning), or abnormal VEP
💎 Board Pearl
The 3 biggest 2024 updates: (1) Optic nerve = 5th DIS region (use MRI orbits, OCT, or VEP), (2) DIT can be substituted by biomarkers (OCBs, kappa FLC, ≥6 CVS lesions, PRL) at a single clinical/radiographic event — DIT is NOT eliminated as a concept, (3) RIS qualifies as MS ONLY when DIS is met AND additional supportive evidence is present (DIT on serial MRI, OR ≥2 CSF-specific OCBs, OR ≥6 CVS/PRL lesions), with no clinical or radiographic alternative. New supportive biomarkers: central vein sign (≥6 lesions or 40%+), paramagnetic rim lesions, and CSF kappa free light chains. Periventricular threshold remains ≥1 (same as 2017, NOT changed).
Diagnostic Criteria & Antibody-Testing Scores
Criterion / ScoreComponentsThreshold / Interpretation
APE2 score (Dubey 2017)
Antibody Prevalence in Epilepsy and Encephalopathy
(1) New-onset refractory seizures, (2) autonomic dysfunction, (3) viral prodrome, (4) psychiatric features, (5) cognitive dysfunction, (6) temporal MRI changes, (7) CSF inflammation, (8) history of autoimmunity or cancerScore ≥4 prompts neural antibody testing (sensitivity ~98%, specificity ~82%)
Graus 2016 — Possible Autoimmune EncephalitisSubacute onset (<3 months) of working memory deficit, altered mental status, or psychiatric symptoms + ≥1 of: new focal CNS findings, seizures not explained by prior disorder, CSF pleocytosis (>5 WBC/µL), or MRI features suggestive of encephalitisAll criteria above + reasonable exclusion of alternative causes = Possible AE; upgrade to Definite when specific antibody identified or other criteria met
Graus 2021 PNS-Care Score
Paraneoplastic Neurologic Syndromes
High-risk phenotypes: encephalomyelitis, limbic encephalitis, rapidly progressive cerebellar syndrome, opsoclonus-myoclonus, sensory neuronopathy, LEMS
High-risk antibodies: Hu, Yo, CV2/CRMP5, Ri, Ma2, amphiphysin, SOX1, KLHL11, DNER/Tr
Scoring 0–10 across phenotype + antibody + cancer + follow-up domains
Definite PNS ≥8; Probable 6–7; Possible 4–5
MOGAD 2023 Criteria(1) Core clinical event (ON, myelitis, ADEM, cerebral monofocal/polyfocal deficit, brainstem/cerebellar syndrome, cortical encephalitis) + (2) MOG-IgG positive by cell-based assay (CBA)clear positive serum titer typically >1:100, OR low/negative serum titer with supporting clinical/radiographic features + (3) exclusion of better diagnosisRequires all three pillars; serum CBA preferred over fixed CBA or ELISA
IPND 2015 NMOSD Criteria6 core clinical characteristics: (1) optic neuritis, (2) acute myelitis, (3) area postrema syndrome (intractable hiccups/nausea/vomiting), (4) acute brainstem syndrome, (5) symptomatic narcolepsy or diencephalic syndrome with typical NMOSD MRI lesions, (6) symptomatic cerebral syndrome with typical NMOSD MRI lesionsAQP4-IgG positive: ≥1 core characteristic + exclusion of alternatives
AQP4-IgG negative / unknown: ≥2 core characteristics meeting strict MRI requirements; ≥1 must be ON, LETM, or area postrema syndrome; dissemination in space required
💎 Board Pearl
APE2 ≥4 = test for neural antibodies. Graus 2016 "Possible AE" is the entry-level umbrella criterion before subtype-specific antibodies define Definite. PNS-Care 2021 replaced the 2004 Graus criteria — uses a numeric score with high-risk phenotype + high-risk antibody + cancer + follow-up. MOGAD 2023: CBA is mandatory; ELISA-only positives are NOT acceptable. IPND 2015 NMOSD: AQP4− cases need stricter MRI criteria + ≥2 cores including ON, LETM, or area postrema.
MS vs NMOSD vs MOGAD
FeatureMSNMOSD (AQP4+)MOGAD
AntibodyNone specific (OCBs in CSF)AQP4-IgG (serum; 70–80% sensitivity by cell-based assay; lower by ELISA ~50–60%)MOG-IgG (serum; CBA preferred)
Sex ratio (F:M)~3:1~9:1~1:1
Age at onset20–4030–50Bimodal: children + young adults
ON featuresUnilateral, mild, retrobulbar, good recoverySevere, often bilateral, posterior > anterior, poor recoveryBilateral, anterior predominant, disc edema, perineural enhancement, good recovery
MyelitisShort segment (<3 vertebral segments), peripheralLETM (≥3 segments), central gray matter, bright spotty lesionsLETM, conus/lower cord predilection
Brain MRIDawson fingers, periventricular ovoid lesions perpendicular to ventriclesOften normal early; area postrema, periaqueductal, diencephalic, cloud-like enhancementFluffy bilateral cortical/deep WM, ADEM-like in children
Spinal MRIShort lesions, dorsolateral, incomplete ring enhancementLETM (≥3 segments), central cord, may enhance diffuselyLETM, conus involvement, can mimic NMOSD
CSF OCBsPresent >95%Usually absent (~15–20%)Usually absent
Relapse patternRelapses then progressive phase (SPMS)Relapsing >90%; rarely monophasic~50% monophasic, ~50% relapsing
Acute treatmentIV methylprednisoloneIV methylprednisolone + early PLEXIV methylprednisolone (very steroid-responsive); PLEX if refractory
Maintenance treatmentDMTs (interferons, anti-CD20, S1P modulators, etc.)Rituximab, eculizumab, satralizumab, inebilizumab, azathioprine, mycophenolateNo FDA-approved DMT; azathioprine, mycophenolate, rituximab if relapsing
MS DMTs harmful?YES — interferons, fingolimod, natalizumab worsen NMOSDLikely — interferons, fingolimod should be avoided
PrognosisVariable; disability accrues over decadesPoor recovery per attack; cumulative disability from relapsesGenerally good recovery per attack; better than NMOSD
Clinical Pearl
Do NOT treat NMOSD with MS DMTs — interferon-β, fingolimod, and natalizumab can worsen NMOSD attacks. Always confirm AQP4 status before starting MS-specific DMTs in patients with LETM or severe/bilateral ON.
💎 Board Pearl
Area postrema syndrome (intractable nausea/vomiting/hiccups) is nearly pathognomonic for NMOSD. MOG-IgG titers can become negative over time — persistent positivity predicts relapsing course. MOGAD optic neuritis shows characteristic perineural enhancement (around the nerve sheath, not just the nerve). NMOSD bright spotty lesions on T2 = high specificity.
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